Journal: Applied and Environmental Microbiology

Article Title: Aspergillus Collagen-Like Genes ( acl ): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection

doi: 10.1128/AEM.02835-13

Figure Lengend Snippet: Basic characteristics of the putative Acl proteins

Article Snippet: The DNA and protein sequence data were analyzed with the Lasergene v.10 software suite (DNASTAR, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 caption a7 Species Pfam designation Acl protein b Name Length (aa) SS a GPI CL region GXY type Location (aa) A. fumigatus 293 Q4WBU6_ASPFU AclF1 359 1–15 GPy 168–244 Q4WW98_ASPFU AclF2 143 GQy 11–106 CBS 144.89 B0Y9H1_ASPFC AclF1 343 1–15 GPy 167–257 B0Y8Y8_ASPFC AclF2 125 GQy 11–88 A. terreus NIH 2624 Q0CXL1_ASPTN AclT1 358 336 GAP 179–262 NIH 2624 Q0CUF0_ASPTN AclT2 265 1–17 GHy 68–196 A. flavus ATCC 200026 B8NLK1_ASPFN AclFL1 554 1–23 GTP 277–366 A. nidulans NCBI 162425 Q5B8U8_EMENI AclN1 432 1–17 411 GQP.GQS 258–315 A. oryzae NCBI 5062 Q2TZV0_ASPOR AclO1 114 GLy 25–110 A. kawachii NBRC 4308 G7XZR3_ASPKW AclK1 239 1–19 GNS.GNR 179–220 A. niger ATCC 1015 G3YG25_ASPNA AclNi1 556 GAT.GKK 484–528 Open in a separate window a SS, signal sequence. b aa, amino acids.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Aspergillus Collagen-Like Genes ( acl ): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection

doi: 10.1128/AEM.02835-13

Figure Lengend Snippet: Primers used for amplification of the acl genes

Article Snippet: The DNA and protein sequence data were analyzed with the Lasergene v.10 software suite (DNASTAR, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 caption a7 Species Pfam designation Acl protein b Name Length (aa) SS a GPI CL region GXY type Location (aa) A. fumigatus 293 Q4WBU6_ASPFU AclF1 359 1–15 GPy 168–244 Q4WW98_ASPFU AclF2 143 GQy 11–106 CBS 144.89 B0Y9H1_ASPFC AclF1 343 1–15 GPy 167–257 B0Y8Y8_ASPFC AclF2 125 GQy 11–88 A. terreus NIH 2624 Q0CXL1_ASPTN AclT1 358 336 GAP 179–262 NIH 2624 Q0CUF0_ASPTN AclT2 265 1–17 GHy 68–196 A. flavus ATCC 200026 B8NLK1_ASPFN AclFL1 554 1–23 GTP 277–366 A. nidulans NCBI 162425 Q5B8U8_EMENI AclN1 432 1–17 411 GQP.GQS 258–315 A. oryzae NCBI 5062 Q2TZV0_ASPOR AclO1 114 GLy 25–110 A. kawachii NBRC 4308 G7XZR3_ASPKW AclK1 239 1–19 GNS.GNR 179–220 A. niger ATCC 1015 G3YG25_ASPNA AclNi1 556 GAT.GKK 484–528 Open in a separate window a SS, signal sequence. b aa, amino acids.

Techniques: Amplification, Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Aspergillus Collagen-Like Genes ( acl ): Identification, Sequence Polymorphism, and Assessment for PCR-Based Pathogen Detection

doi: 10.1128/AEM.02835-13

Figure Lengend Snippet: acl-based detection of non-A. fumigatus species. (A) PCR amplification and gel electrophoresis of various acl genes among non-A. fumigatus species. The species (from top to bottom) and assays are shown as follows: A. flavus (amplification of the 403-bp aclFl1-3′ region; A. flavus-specific amplicons are marked with arrowheads, and an amplicon detected in A. parasiticus is marked with an asterisk); A. nidulans (amplification of the 227-bp aclN1-3′ region); A. niger (amplification of the 297-bp aclNi1-5′ region); and A. terreus (amplification of the 262-bp aclT2-5′ conserved region). PCR amplification was resolved by 2% agarose gel electrophoresis. M, 1-kb Plus TrackIt DNA ladder. (B) Nanogel electrophoretic separation. PCR amplicons specific for A. fumigatus, A. flavus, A. nidulans, A. niger, and A. terreus were simultaneously resolved and detected with the intercalating dye SYBR green 1. The separation is accomplished using a 25-μm-inner-diameter capillary with an effective length of 40.2 cm, E = 100 V/cm, 30°C, and a 10% nanogel with [DMPC]/[DHPC] = 2.5 at 6 kV for a 2-s injection. RFU, relative fluorescence units.

Article Snippet: The DNA and protein sequence data were analyzed with the Lasergene v.10 software suite (DNASTAR, Inc., Madison, WI). table ft1 table-wrap mode="anchored" t5 caption a7 Species Pfam designation Acl protein b Name Length (aa) SS a GPI CL region GXY type Location (aa) A. fumigatus 293 Q4WBU6_ASPFU AclF1 359 1–15 GPy 168–244 Q4WW98_ASPFU AclF2 143 GQy 11–106 CBS 144.89 B0Y9H1_ASPFC AclF1 343 1–15 GPy 167–257 B0Y8Y8_ASPFC AclF2 125 GQy 11–88 A. terreus NIH 2624 Q0CXL1_ASPTN AclT1 358 336 GAP 179–262 NIH 2624 Q0CUF0_ASPTN AclT2 265 1–17 GHy 68–196 A. flavus ATCC 200026 B8NLK1_ASPFN AclFL1 554 1–23 GTP 277–366 A. nidulans NCBI 162425 Q5B8U8_EMENI AclN1 432 1–17 411 GQP.GQS 258–315 A. oryzae NCBI 5062 Q2TZV0_ASPOR AclO1 114 GLy 25–110 A. kawachii NBRC 4308 G7XZR3_ASPKW AclK1 239 1–19 GNS.GNR 179–220 A. niger ATCC 1015 G3YG25_ASPNA AclNi1 556 GAT.GKK 484–528 Open in a separate window a SS, signal sequence. b aa, amino acids.

Techniques: Amplification, Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SYBR Green Assay, Injection, Fluorescence